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"But when they switched back to normal operations, the system did not come up".
The perfusion buffer was switched back to normal aCSF and three additional samples were collected.
When LNCaP androgen-depleted for 42 days were switched back to normal FBS medium, cell growth resumed over the period of the following week and, within the next 21 days in FBS medium, the cell morphology reverted to that of parental LNCaP cells (data not shown).
Then neurons were switched back to normal culture conditions for 3 or 6 h before being harvested.
Confluent MDCKII cell cultures were first incubated in calcium-free medium for 6 h, then switched back to normal medium and processed for immunofluorescence to detect E-cadherin and vezatin at different times.
Similar(55)
"With refiners late in switching back to normal gasoline yields, we project gasoline cracks will be strong this year".
The shocks were given continuously for a period of 4 days before switching back to normal nitrophenolic loading (200 mg/L d).
But the company ultimately decided to switch back to normal pricing for customers, while continuing to pay drivers twice as much as normal rates to keep them on the road.
Once their careers are done, they often struggle to switch back to normal eating habits, and deal with weight problems for the rest of their lives.
In these studies, 2DG application results in a decreased fEPSP slope, which is reversed upon switching back to normal ACSF leading to potentiation.
From day 3 on growth medium was only supplemented with 1 μg/ml insulin for 2 days before switching back to normal growth medium.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com