Sentence examples for switch specificity from inspiring English sources

The mutations thus broaden rather than switch specificity.

However, BsLDH likely is an exception in the LDH/MDH superfamily, since the reverse mutation (Arg102Gln) fails to switch specificity in MDHs from two other species (Nicholls et al., 1992; Cendrin et al., 1993).

In SCR, a domain swapping experiment in Brassica between allele SCR6 and allele SCR13 resulted in a switch from SCR6 to SCR13 specificity, but failed to switch specificity when the reverse swapping (SCR13 domains to a SCR6 backbone) was performed [ 9].

Modification of the hapten type in coating conjugates applied in present study served as a mechanism for switching specificity of the ELISA between selective and group.

In Haloarcula marismortui (Hm) MDH, the Arg102Gln mutation switches specificity, but the mutant's kcat/KM for pyruvate is 200-fold less than the wild-type's kcat/KM for oxaloacetate.

In the apicomplexan dehydrogenases, the evolutionary mutations that switched specificity from oxaloacetate to pyruvate (the six-residue insertion and Arg102Lys) are insufficient to confer pyruvate activity in modern apicomplexan MDHs (PfMDH-R102K, PfMDH-INS, CpMDH-INS, PfMDH-R102K-INS, Figure 4).

This may be most evident from the observation that downstream rewiring mutations can lead to a switch of specificity of a comparable magnitude to the specificity difference between two distinct kinases in the human kinome.

One of these showed an additional switch in specificity by introducing favorable interactions between a designed arginine in the peptide and phosphoserine on the PDZ domain.

In order to analyze the structural changes involved and to more fully understand the basis of this switch in specificity, we have isolated all 20 variants of the enzyme at position 192 and determined the activities with a range of substrates.

Mutation of His194 to threonine proved most significant, with a switch in specificity from NADH to NADPH [ kcat/Km NADH /kcat/Km NADPH) from 1.5 1 to 1 3.5, mostly as a result of a reduced Km for NADPH of approximately sevenfold in the His194Thr mutant.

We then introduced either single or double mutations at Q226L and G228S in the SA binding site of H5 HA [21] equivalent to those previously shown to switch receptor specificity in H2 and H3 HA subtypes [8].