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Our finding of increased expression of interferon-associated host genes in clinical samples from patients with 2009 H1N1 infection is intriguing and consistent with very recent data showing that the NS1 protein of 2009 H1N1 lacks the ability to block general host gene expression in both human and swine cells in vitro [47].
Within minutes swine cells or organs transferred to non-human primates, such as baboons, are destroyed.
The Cre-recombinase system has been confirmed to be functional in swine cells under physiological conditions (Wells, unpublished).
In addition, swine cells also contain sialyl α2,3-galactose-linkage, the receptor for avian influenza viruses.
Swine cells express sialic acids that can be receptors for swine, human, and avian influenza strains and facilitate cross-species influenza transmission and the genesis of novel influenza strains.
In order to utilize swine as a model for xenotransplantation, one of the first obstacles was to remove the α-gal epitopes on the swine cells by disruption of GGTA1[ 13, 65- 72].
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Swine may play roles as intermediate hosts for influenza viruses between humans and avian species because swine cell receptors have both the α2,6- and the α2,3-linked sialic acid side chains on their surface glycoproteins (Ito and Kawaoka 2000).
The A(H1N1 pdm09 virus was not able to block host gene expression in both human and swine cell lines, which is partially due to mutations that block CPSF30 binding.
We observed such convection current-induced disturbance of the infection spread in the case of some cell types used in preliminary experiments, such as swine testis cells (ST) or canine (MDCK) and human kidney cells (HEK293) (data not shown).
Coronaviruses interacting with the cell membrane or inside the cell cytoplasm were easily seen when grown in swine testicle cells.
Indirect immunofluorescence assay (IFA) was performed using commercial IFA slides for detection of PCV2-infected (virus strain T-657) swine testicle cells (PT-1) and PCV1-infected PK cells using commercially available slides (VMRD, Inc, Pullman, WA, USA) as recommended by the manufacturer.
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