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Visual observation ensured that no erratic swimming behaviour or premature fatigue occurred during the swimming trial.
Each swimming trial was subjectively judged as good or bad.
Immediately after the swimming trial the mice were injected intraperitoneally with ISRIB (0.25 mg/kg in saline, 1% DMSO).
Mice were handled daily for 3 days before the experiment, and the training protocol consisted of one swimming trial per day.
An oval shaped stream-gutter (6.0*4.0*0.8 m; 6,000-l) was constructed to enable a 3-months swimming trial.
We quantified swimming performance for each individual hatchling turtle by using LabChart v7.0 to calculate mean thrust (mN), stroke rate, proportion of time spent power-stroking and mean thrust per power stroke for each 10-min period throughout the 4 hour swimming trial, as per Pereira et al. (Pereira et al., 2011).
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