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Swimming assay was performed as previously described by Rashid and Kornberg [ 18].
Only transformants showing motility in the swimming assay showed the two bands corresponding to the RSP3 protein.
A higher duration of immobility in the forced swimming assay was found in APP/PS1 mice.
Aliquots (2 μL) of culture suspensions were drop-inoculated (swarming assay) or sink-inoculated (swimming assay) onto plates and air-dried in a laminar-flow cabinet.
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Yet, the majority of state-of-the-art image analysis systems are designed for a specific environment, most commonly crawling [27], [30] [34], [37] or swimming assays [29], [39].
For swimming assays, LB grown B. cenocepacia cultures (A600 nm = 1.0) were inoculated with a toothpick on 'swimming plates' (1 g/l tryptone, 0.5 g/l yeast extract, 5 g/l NaCl, 3 g/l agar noble) and incubated for 42 hours at 37°C.
Remarkably, vt25 animals displayed a lack of sensitivity to the osmolarity of solutions used in swimming assays.
For bulk swimming assays, worms were placed in an open petri dish (6 cm dia).
Studies of C. elegans' motility behavior now include various swimming assays [13], [15] [19], as shown in Fig. 1(b)–(d) and (g).
For the swimming assays, bacterial cultures grown overnight in SB at 28°C were centrifuged and resuspended in PBS at the same OD600, 200 µl of each suspension were centrifuged and the pellets resuspended in protein sample buffer.
Both revertants were nonmucoid, and determined to be motile by swimming motility assay (Figure 6, A and B).
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