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where γ 1 = γ 2 = 0.0001, and f ̂ 1 = 0. 12 and f ̂ 2 = 0. 1044, so that the difference between the frequencies of cisoids is kept constant (same as the stationary signal case) during frequency sweep interval, and then according to the random walk model of[9] in the statistical analysis subsection, f k ( n ) = f k ( n − 1 ) + γ k. φ k ( n ), k = 1, 2, and n = 2, …, N (95).
The sweep interval between each current step was of 10 sec.
Secondly, in [ 30] all constants values have a reference to experimental measurements, except for the constant of this reaction (which corresponds to entry 29 in Table 1 in [ 30]). Figure 6 shows the results of this PSA-1D, where k44 was varied over six orders of magnitude, considering the value given in [ 30] as the upper limit of the sweep interval.
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To balance the effects of inactivation, slow recovery, and rundown, we selected 350 ms pulses for the current-voltage protocol, and an inter-sweep interval of 20 s.
Inter-sweep interval was 10 s to allow for current de-inactivation.
For multiple spiking measures, we injected 500-ms current steps of increasing amplitude with 2-s inter-sweep interval until the neuron passed its maximum spike number.
As seen in Fig. 9c and d, recovery from I Ba inactivation was indeed very slow (~ 120 s) and was twice as long as the inter-sweep interval in our steady state protocol.
For AP waveform measures, we injected 10-ms current steps of increasing amplitude with 2-s inter-sweep interval until reaching the current threshold at which the neuron reliably fired an AP (Vm > 0 mV).
Frequency sweep time interval was set to 26 seconds, corresponding to an increase rate of about 1.7 Hz per second.
Data acquisition and command potentials were controlled with a commercial software program (Patchmaster, HEKA) with a sweep time interval of 1 s and a sample rate of 200 kHz.
The genomic coordinates of each SNP, together with its MAF, are available in Additional file 2. The values in the parentheses of swept radius are confidence interval of swept radius.
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CEO of Professional Science Editing for Scientists @ prosciediting.com