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To generate the CD36 ECFP fusion we adapted the sequence swapping protocol proposed by the Quikchange XL mutagenesis kit [38].
This paper proposes an architecture and communication protocol called the Slot Swapping Protocol which is used to interconnect, by means of a backbone, different subsystems that generate and consume real-time data.
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Phylogenetic trees were inferred using the GARLi program (Genetic Algorithm for Rapid Likelihood Inference) [18], which employs an extensive branch-swapping protocol and optimizes the substitution model iteratively during the search.
All head kidney samples were analyses using the dye swap protocol.
Each of the 6 replicates was hybridized using a dye swap protocol resulting in 12 slides per group and 48 slides in total.
RNA was reverse transcribed using a FAIRPLAY II cDNA post labelling kit (Stratagene) according to the manufacturer's instructions and labelled separately with both Cy3 or Cy5 (GE HealthCare; PA23001, PA25001) to allow for a swap protocol.
For the Invitrogen microarray, RNA samples were labeled using a two-color and dye-swapping protocol.
The Invitrogen array was tested using a two-color and dye-swapping protocol so that, at first, two RNA samples were divided and differently labeled in red-green and green-red combinations, and each combination of the RNA sample set was simultaneously hybridized to a microarray.
The first is a major problem when developing time sensitive protocols (such as TDMA, fast software acknowledgements,...) while the second prohibits swapping or modifying protocols at run-time.
Left to sweep up the discarded popcorn of departed customers, Sam Mattheww Maher and Avery Aaron Clifton Motenen) pass the hours swapping movie trivia, reviewing protocol, and tiptoeing around their attraction to Rose (Louisa Krause), the punky projectionist.
Nodes agree on a total ordering for processing requests with a novel low overhead wait-free algorithm that utilizes a small amount of shared memory accessible to the nodes and a simple compare-and-swap like protocol which allows the system to progress at the speed of the fastest node.
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