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This normalization approach eliminates the need for dye swap experiments with two-color arrays.
Finally, we performed promoter swap experiments with CPC and TRY and tested the ability to rescue the try mutant trichome phenotypes.
To address the reviewer's concerns about a co-purifying bridging factor, we have repeated the in vitro charge swap experiments with telomerase produced and purified from rabbit reticulocyte lysates (RRL).
To further understand the molecular nature of the uniqueness of TRY among the six R3-single repeat MYB inhibitors, we used promoter swap experiments with CPC which shares all aspects of the TRY expression pattern as judged by promoter:GUS analysis.
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Each pool of precocious brains was compared in a dye swap experiment with each pool of non-precocious brains.
The microarray results are based on the geomean of ten normalized expression values derived from three biological replicates and two dye-swap experiments with a technical replicate for each array.
Furthermore, to rule out the possibility of a bridging factor that co-purifies with telomerase from HEK293 T cells, we carried out the charge-swap experiment with telomerase purified from rabbit reticulocyte lysates (RRLs).
Four independent dual colour hybridisations including two dye swap experiments were performed with RNA from each of the four transfected cell lines (in total n=16) using non-transfected cells as reference.
A double loop microarray design with dye swap experiments was adopted to profile the transcriptome of developing Pima and TM1 fibers independently.
Using a quantitative model of the regulatory network we were able integrate the wealth of experimental knowledge about the S. cerevisiae GAL network with promoter swap experiments and to infer and simulate the operation of an ancestral form of the GAL network.
This resulted in functional proteins, consistent with the previous domain swap experiments, which showed that the extensin domains of LRX1 and LRX2 are interchangeable [ 26].
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