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Exact(27)
cDNA microarray analysis of peri-infarct tissue was done using a custom cloneset and employing a looped dye swap design.
Interestingly, the swap design of counter flow arrangement (parallel channel or wavy) shows two maximum temperatures in the first and second half of the heat sink.
A two-color 12×2 paired swap design [74] using 24 arrays was applied, comparing RNA samples from 12 independent allogeneic and 12 independent syngeneic skin explant assays.
For each time point, RNA from 5 mice was pooled and hybridized in quadruplicates, using a dye swap design to produce 4 expression measurements per time point.
The second one was a dye swap design as follows: C1 in the green channel against V1 in the red channel; V1 in the green channel against C1 in the red channel; C2 in the green channel against V2 in the red channel; V2 in the green channel against C2 in the red channel.
A dye swap design of hybridization was applied.
Similar(33)
Microarray hybridization experiments were performed by NimbleGen Systems using a duplicated dye-swap design.
Six independent biological replicates were used per treatment to produce six replicate microarrays per experiment in a dye-swap design.
The data processing framework was mainly based on snapCGH [52], and some necessary modifications were made to fit the requirements of quality control and the dye-swap design.
Briefly, 20 µg of RNA were converted to cDNA, labeled with Cy-3 or Cy-5 dye and hybridized together with common reference RNA in a dye-swap design.
A dye-swap design was used.
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