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We used the parsimony criterion and tree-bisection reconnection to swap branches in the tree space with PAUP * 4b10 for 100 bootstraps [ 37], with a hetequal character transition matrix, as previously described by James et al. [ 38].
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The second way to alter a tree structure is by swapping branches of trees on the workbenches.
Each phylogenetic tree was bootstrapped 100 times allowing for the global swapping of branches.
Branch Swap Filter: Very Strong.
The bootstrap value of the node that groups many of the newly described RNA polymerase inteins into a cluster distinct from all other inteins ranges from 70% (fast heuristic search, no branch-swapping) to 99% (heuristic search with branch-swapping).
Heuristic searches were done with one random addition sequence replicate and TBR branch swapping; 1,000 bootstrap replicates were performed without branch swapping.
ML and MP analyses were performed using PAUP* 4.0b10 [ 56] with ten random stepwise heuristic searches and tree bisection-reconnection (TBR) branch swapping (for MP), or subtree pruning-regrafting (SPR) branch swapping (for ML).
Bootstrapping was determined using 10,000 replicates, MULTREES option off, TBR branch swapping, and five random addition sequences.
Tree bisection-reconnection (TBR) was used for branch-swapping.
A heuristic search was then performed using SPR branch swapping.
Tree bisection and reconnection (TBR) was utilized as the branch-swapping algorithm for the heuristic search.
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