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Swabs were placed in collection media (M4-RT; Micro-Test, Lilburn, GA).
The swabs were placed in a cryovial and stored at −20°C until analysis.
Two swabs were placed in Cary-Blair transport media and one swab in Campy-Thio-Broth.
Oropharyngeal swabs were placed in transport media and stored at −70°C until they were delivered on dry ice to NMRCD for laboratory analysis.
The cotton swabs were placed directly into 1.5 ml plastic tubes as per [12], and the adhesive tape was stuck onto microscopic slides as per [13].
Briefly, nasal swabs were placed in trypticase soy broth (TSB), vortexed for 15 seconds and placed in 8 ml of phenol red mannitol salt broth (PHMB).
These swabs were placed in sterile tubes containing 500 µl of sterile PBS/0.05% Tween-80, sealed, and transported on ice to a lab within 3 4 hours of collection.
The swabs were placed in 1 mL of virus transportation medium (Hanks balanced salt solution) as described in Wallensten et al. [7] and kept on ice until they were stored at −80°C.
The swabs were placed in 2-ml tubes with virus transport media [Hanks Balancedd Salt Solution containing 0.5% lactalbumin, 10% glycerol, 200 U/ml penicillin, 200 µg/ml streptomycin, 100 U/ml polymyxin B sulfate, 250 µg/ml gentamicin, and 50 U/ml nystatin (ICN, Zoetermeer, Netherlands)] that were immediately frozen at −70°C (at the latest, 30 min after sampling).
Swabs were placed in SurePath® media.
Swabs were placed in liquid thioglycollate broth for primary culture.
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