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All swabs were kept frozen at -20°C.
The swabs were kept in Amies Transport Medium (Copan, Italy) and processed within 5 hours from sampling.
On June 21st and 22nd, 2003, 62 birds were sampled, and the swabs were kept refrigerated at 2 8°C until June 23rd before being sent by mail to the laboratory where they were received the next day for examination.
Samples were obtained from the birds in the same way as with the graylag geese on Smøla (three cloacal swabs), and the swabs were kept on ice for up to two hours before they were frozen at -70°C.
The swabs were kept on ice and transported to the laboratory within 1 4 h, and were then processed for further analyses, while the vaginal smears were transported dry.
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The second swab was kept in one of the Kalon TV latex tubes included with the kits containing sterile PBS, pH 7.2, to perform the latex agglutination.
The first swab was kept in tube containing 3 ml sterile Phosphate Buffered Saline (PBS), pH: 7.2, for wet mount microscopy and Giemsa staining.
Then three vaginal swabs were collected from the posterior fornix of the vagina by a sterile cotton wool swab sticks with wooden shafts to be examined as follows: The first swab was kept in tube containing 3 ml sterile Phosphate Buffered Saline (PBS), pH: 7.2, for wet mount microscopy and Giemsa staining.
Swab results were kept undisclosed until the end of the study.
All swab specimens were kept frozen at -20°C until tested and all cerebrospinal fluids were frozen at -80°C until tested.
The vaginal swab specimens were kept in Copan UTM transport medium and refrigerated for up to 5 days before shipment at ambient temperature, typically reaching the laboratory within 1 to 2 days.
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