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Genomic DNA was extracted from buccal swab using a QIAamp DNA Minikit (QIAGEN, Hilden, Germany).
Then, 100 μL of this saliva stock, containing 132,100 nucleated human cells, was added to each swab using a Gilson P200 Pipetman.
Women who are sexually active are asked to provide a self-collected vaginal swab using a swab kit sent in the post.
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Instead of using a q-tip or cotton swab, use a cotton ball because they are softer and won't hurt sensitive ears.
The cervix and ectocervix were swabbed using a circular motion.
The screen, sides, and back of mobile phones were swabbed using a sterile swab.
The study was based on molecular screening of cloacal and tracheal swabs, using a pan-influenza A RT-PCR.
DNA was extracted from the cheek swabs using a phenol chloroform method (see Additional file 1 for the full extraction protocol).
Women receiving HPV testing were required to attend their clinic for a swab using the Digene Hybrid Capture 2 HPV DNA test (Gaithersburg, MD, USA).
Genomic DNA was extracted from swab samples using a bead-beating and enzymatic lysis protocol, followed by purification using a QIAamp DNA Mini Kit (Qiagen, Valencia, USA).
DNA was extracted from buccal swab samples using a QIAamp DNA Mini Kit (Qiagen, Hilden, Germany).
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