For detection of Brachyspira in dry swab extracts, the nox PCR described above was used.
Virus titres in nasal swab extracts and serological responses were also evaluated.
The results of virus titres of the nasal swab extracts collected from the dogs are presented in Table 3.
Prospectively, we collected the same information from patients tested for influenza by rapid test kit and used nasopharyngeal swab extracts from rapid test kits for virus typing.
To detect enteroviruses, including EV75, in throat swab extracts, RNA was extracted by using the Chemagic Viral DNA/RNA kit (Chemagen AG, Baesweiler, Germany) and a Kingfisher mL Magnetic Extractor (Thermo Fisher Scientific Inc., Waltham, MA, USA).
To investigate the performance of direct genus-specific nox PCR on rectal swab extracts relative to culture, culture positive samples that had been successfully sub-cultured and identified by nox gene sequencing (n = 81) were matched with a set of culture negative samples.
Once they receive the swabs, they extract genetic material from the cells and, in the case of the Y chromosome test, will analyse between 10 and 60 or so segments of the DNA.
Contamination controls in the form of sterile swabs were extracted after every fifth swab processed in the DNA extraction robot.
For real-time J.B.A.I.D.S. PCR, cycling was carried out in a J.B.A.I.D.S. real-time thermocycler (Idaho Technologies, UT, USA) using 1 µl (∼1.8 µL of throat swab) of extracted DNA in 10 µl of TaqMan Universal PCR Master Mix (Roche), containing 5 mM MgCl2 and 400 nM forward and reverse primers.
100 μL of each NP swab were extracted using the UltraClean® Microbial DNA Isolation Kit (Mo-Bio, USA) following manufacturer's protocol.
