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After plating, the remaining swab content in STGG was then frozen for future use at −70°C.
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Standard microbiology culture and identification techniques were used to analyse the swab contents for the presence of S. pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, S. aureus, Pseudomonas aeruginosa and Neisseria meningitidis.
Multiple-site testing for fungi included tracheal secretions, swab, stomach contents, pharyngeal, rectal and groin skinfold swabs, urine, and blood.
Investigators plan to take skin and mouth swabs, stomach contents, slices of organ tissue and vials of bile from animals that have died and test them for disease and hydrocarbons, as well as for dispersants, before a final report on the cause of death is written.
In contrast, Petersen et al. [ 14] and Herman et al. [ 15] reported no findings of Campylobacter from different samples collected in the hatchery e.g. incubator content, swab samples from hatchery machinery and floors and yolk sacs of diseased or dead chicks.
The number of cells in adherent monolayers was determined by a series of steps: adding 100 μL PBS into each well, scraping the wells with sterile pipette tips, swabbing the wells with a cotton-tipped swab, mixing the contents, and pipetting 10 μL of the content for viable counts.
Upon admission, rectal swab with visible stool content was collected for the detection of multidrug-resistant organisms including VRE in accordance to the hospital infection control policy.
The bacterial load of VRE in rectal swabs with visible stool content was monitored daily.
Bd contents of swab samples taken the day before of death of the two treated newts were 0 and 14 BdGE in female and male individual, respectively.
Infection was detected by quantitative amplification of Bd-DNA content of weekly swab samples (from: forelimbs, hind limbs, abdomen and cloaca) using fine tip dry swabs (Tubed sterile dryswabTM tip, Medical wire & Equipment, cat. No MW100).
In order to evaluate to what extent faeces and rectal swabs differed in regard to content of human cells we analysed 24 faeces and 24 rectal swab samples by real-time PCR targeting betaglobin, a human gene.
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