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A mouse was attached by the tail to a horizontally mounted force transducer using suture silk and subsequently placed in an apparatus that allowed only forward movement.
The proximal tendon was attached to 4-0 silkre silk to a dual-mode muscle lever system (model 300B-LR Aurora Scientific, Aurora, ON, Canada).
The mesorchium of each testis was ligated using 2 0 suture silk and each testis was removed, leaving the fat bodies intact.
Once the surgical dissection of the muscle under study was complete, a marker tie was made in the insertion tendon (using 2 0 braided suture silk; CP Medical MedRep Expresss, Prescott, AZ) as close to the junction of muscle fibers and tendon as possible.
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Sutures: 3 0 silk suture (Ethicon, Somerville, NJ, USA), covered with a bordered IV cover dressing (3M™ Tegaderm™ Transparent Film Dressing with Border cat.1655, 3M Company, St . Paul MN, USA).
1300 participants with major trichiasis were randomised to absorbable polyglactin-910 sutures or silk sutures and followed-up at one year and two years.
Following placement of the clip, the clip was sutured with 5 0 silk suture thread, the intestine returned into the abdomen, and the abdomen sutured shut.
Gingival formers were not covered with soft tissue and mucoperiosteal flaps were sutured with 3/0 silk suture material.
For the repeat imaging experiments, the plate was carefully detached from the skull and the scalp was sutured (Ethicon 6-0 sutureuture).
The fascia was sutured using 3 0 silk suture and the skin closed with wound clips.
The flap was then repositioned and sutured with 4 0 silk suture.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com