The results show that persistence of YY1 expression in cardiac progenitor cells (CPCs) can help sustain these cells in a primitive state while the loss of YY1 expression results in rapid differentiation of CPCs into cardiomyocytes, endothelial cells, and smooth muscle cells.
Based on the culture morphological analysis and gene expression analysis the N2 medium enhanced more growth of salivary gland epithelium than mesenchyme but was only able to sustain these cells for a short period whereas the DMEM plus serum effectively promoted the proliferation of both salivary gland epithelium and mesenchyme in long-term culture.
To investigate the molecular expression of the receptor-ligand pair that comprise the essential paracrine signaling pathway sustaining these Wnt1-induced cell populations, we assayed mRNA expression for Wnt1 ligand, Lrp5 receptor and axin2, a Wnt reporter, in the separated cell types.
Therefore, there is a need to construct a synthetic microvascular network, which would sustain these cultured cells in a similar manner to normal tissue.
Although HRG-induced expression was generally of a lower magnitude than for EGF, it was often sustained compared to EGF, consistent with our previous finding that HRG-dependent mitogenic signalling is sustained in these cells [ 32].
The large quantity of DNA damage sustained by these cells due to checkpoint abrogation results in mitotic catastrophe and subsequent cellular death from this mitosis.
Bhatia's challenge was to find a way to sustain the cells within a mechanical cartridge.
Matrices of supportive, fibrous proteins sustain the cells of the heart, lungs, and other tissues in the body.
To determine whether the metal complexes have sustained effects on these cells, following their removal, the cellular recovery was assessed within a 24 h period post replacement of the metal complex medium with fresh media.
While the defined and serum-free AECGM was sufficient to culture both primary and passaged EBECs on solid supports, this same medium was not suitable for sustaining proliferation of these cells on membrane insert that allow differentiation of EBECs.
