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Our current data show that an optimal combination of cis-acting elements and promoters/enhancers for sustaining gene expression in chicken cells is suggested.
This demonstrates that bZIP1 is directly or indirectly involved in sustaining gene expression modulation by light, as confirmed by previous studies [ 12].
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This leads to a rapid and sustained gene expression, thus, physical delivery systems represent a convenient and efficacious method for gene delivery in vivo.
Here, we test the ability of these vectors to sustain gene expression and phenotypic improvement for the life of the animal.
Recombinant retroviruses provide highly efficient gene delivery and the potential for sustained gene expression, but suffer from significant disadvantages including low titer, expensive production, poor stability and limited flexibility for modification of tropism.
We have recently reported the design of hybrid delivery vectors combining retrovirus-like particles with synthetic polymers or lipids that are efficient, provide sustained gene expression and are more stable compared to native retroviruses.
This situation suggests that, unlike other HP1 isoforms, HP1γ may sustain gene expression in ESC.
All these findings indicate that autologous cell gene delivery can make sustained gene expression in the injected area locally.
Numerous cellular/viral promoters that ensure sustained gene expression in various cell types have been identified but systematic comparison of their activities in hESCs is still lacking.
These results showed the feasibility of inducing a sustained gene expression in bones following injections of an HVJ-envelope-DNA complex vector into the muscle.
In conclusion, we have demonstrated that the injection of an HVJ-envelope-DNA complex into an innervated muscle can achieve efficient gene expression in the interior of bone, and that sustained gene expression can be obtained by repeated gene transfers.
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