Complete frataxin deficiency in this fibroblast cell line does not sustain cell division and survival [30].
These results suggest that FipA is required to sustain cell division in bacteria subjected to oxidative stress.
In addition, our results show for the first time that complete frataxin deficiency in a fibroblast cell line does not sustain cell division and survival.
Macrophages can extensively proliferate in vitro when stimulated with macrophage growth factor, which is present in most tissues at concentrations sufficient to sustain cell division [6], [7], [8].
Using a fluorescent Cre recombinase in fibroblasts carrying the conditional frataxin allele, we first demonstrated that complete frataxin deficiency in a fibroblast cell line does not sustain cell division and survival.
However, these reports do not provide any indication of variability or reproducibility of the protocol, and the isolated protoplasts did not display sustained cell division.
This isolation system has facilitated recovery of viable protoplasts capable of rapid cell wall re-synthesis and sustained cell division to form callus. Further, isolated protoplasts survived electrofusion and viable heterokaryons were produced.
Together, these results provide the first evidence of sustained cell division, callus regeneration, and potential application of somatic cell fusion in American elm, suggesting that this source of protoplasts may be ideal for genetic manipulation of this species.
The reconstructed cells can then be analyzed for loss of the senescent characteristics of the donor nuclei (Fig. 1; boxes 1 and 2) and whether the 'age reprogrammed' nuclei can trigger re-entry of the reconstructed cells into the cell cycle and sustain cell division.
Dexamethasone-induced PLT2 expression in ABP1 inactivated roots did not reactivate cell division (Figure 5), coherent with previous data supporting that PLT overexpression sustains cell division only in cells that still have the capacity to divide [22].
