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These G418 and metal concentrations were then maintained continuously, providing sustained selection pressure and promoter induction.
To assess the potential effects of this sustained selection pressure, 127 Aeromonas spp. isolated from warm and cold water ornamental fish species were screened for tolerance to 34 antimicrobials.
Stable cell lines were obtained by sustained selection with G418.
Footprints of sustained selection in livestock are generally diverse and complex.
Since then, sustained selection has further increased muscle mass of Belgian Blue animals to a comparable extent.
Our results suggest that at least the SAMD9 genes may have been under sustained selection pressure exerted by viral pathogens.
Similar(49)
Sources of polymorphism other than standing variation must therefore have contributed to sustain selection during 16 generations.
To sustain selection pressure, low-passage-number HEK293-gfpPLD2 cells were maintained in DMEM supplemented with 10% FBS, 100 μg/mL penicillin streptomycin, 2 μg/mL puromycin, and 600 μg/mL G418.
As noticed already by Mayr (1963), sustained disruptive selection, operating over many generations, is only achieved under highly restrictive and unrealistic conditions in classical population genetical models.
Because high variation at immunity genes appears to be sustained by selection, surveys of intraspecific variation at such loci have been employed in ways that neutral markers cannot be.
This was predictable, because two forces that are both expected to erode genetic variability – random genetic drift and sustained directional selection – are likely to be pronounced in laboratory cultures, particularly given that effective population sizes are likely to be much lower than those occurring in natural populations of Drosophila.
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