The electric field is able to fracture mechanically sustained samples.
Then, for case 1 (T vs. S), transient samples are collected as those satisfying the criterion that the ERK level at the last time-point observation (i.e., at 60 min) is less than 10% of the maximum amplitude; sustained samples are collected according to the maximum duration, in addition to considering the maximum amplitude.
Because the maximum amplitude of ERK often occurs within the first 10 to 20 min (within the 60 min period), we assumed sustained samples would have the duration of more than 30 min; accordingly, samples of the duration of less than 30 min have been discarded.
The purpose of this study was to compare microphones of different directionality, transducer type, and cost, with attention to their effects on acoustical measurements of period perturbation, amplitude perturbation, and noise using synthesized sustained vowel samples.
Typical responses begin 10 20 ms after stimulus onset and reach a phasic peak within the first 50 ms. The sustained window samples the post-peak plateau region of the response and was originally chosen so as to be able to measure responses to short stimulus durations.
Challenges noted during the initial studies included (a) difficulty in achieving optimum dilution and residence time while sustaining isokinetic sampling for high flue gas velocities; (b) inaccuracies in measurement and control of sampling system flow rates to maintain a balanced flow system; and (c) particle depositions in several system components.
Similar to the qRT-PCR experiments, virus titers were higher in tissue samples than in swab samples but were not as sustained; all tissue samples were positive at day 3 posteuthanasia but negative by day 4.
Statistical model equations directed production of a control batch that minimized span, maximized yield, and targeted a t50 of 90 min (sample A); sample B that differed by targeting a t50 of 240-300 min to provide sustained release; and sample C that differed from sample B by maximizing span.
The amount of variability over time and space has not been fully characterized and would be difficult to characterize without a sustained and major sampling effort.
The new system also has the ability to sustain multiple samples during each experimental run.
All samples sustained macrophage adhesion and growth.
