Both of these properties are crucial for achieving sustained expression of the transgene.
Lentiviral vectors transduce both dividing and non-dividing cells and can support sustained expression of transgenes.
Seiler, M.P. et al. Elevated and sustained expression of the transcription factors Egr1 and Egr2 controls NKT lineage differentiation in response to TCR signaling.
Such long-term survival represents an important first step toward developing an optimal cell-based delivery system that generates sustained expression of a therapeutic molecule.
However, long-term, high-level, and sustained expression of angiogenesis inhibitors, such as angiostatin, is necessary to prevent dormant tumors from becoming active again.
Limiting the lethality inherent in the Sindbis virus vector in order to enable long term, sustained expression of recombinant proteins may be possible.
At 5 hpf, embryos injected with miR-430 morpholino exhibited sustained expression of genes that are normally downregulated at MZT, suggesting the effectiveness of the morpholino (Supplementary Fig. 4a).
In conclusion, we show that menin functions as a global regulator of the stress response promoting sustained expression of heat shock genes.
Pseudotyped lentivirus carrying shRNA can be efficiently integrated into the chromosome, making it a good carrier for the delivery and sustained expression of shRNA [5], [28].
Coincidentally, only PVD and FLP showed sustained expression of DMA-1(Liu and Shen, 2012).
Interestingly, sustained expression of FLAG-MCL-1 mediated resistance to the proapoptotic effects of flavopiridol.
