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Both of these properties are crucial for achieving sustained expression of the transgene.
Lentiviral vectors transduce both dividing and non-dividing cells and can support sustained expression of transgenes.
Bhattacharyya, S. et al. Smad-independent transforming growth factor-β regulation of early growth response-1 and sustained expression in fibrosis: implications for scleroderma.
Increasing RGD density increased expression levels up to 100-fold, while greater solids content sustained expression levels for 16 days.
Such long-term survival represents an important first step toward developing an optimal cell-based delivery system that generates sustained expression of a therapeutic molecule.
A gene therapy-mediated approach to the delivery of antiangiogenic agents using adeno-associated virus (AAV) vectors has a number of advantages including the potential for sustained expression.
However, long-term, high-level, and sustained expression of angiogenesis inhibitors, such as angiostatin, is necessary to prevent dormant tumors from becoming active again.
Here we test the ability of the AAV1-F9 vector to deliver sustained expression and correction of factor IX (FIX) deficiency in genetically engineered hemophilic mice.
Limiting the lethality inherent in the Sindbis virus vector in order to enable long term, sustained expression of recombinant proteins may be possible.
At 5 hpf, embryos injected with miR-430 morpholino exhibited sustained expression of genes that are normally downregulated at MZT, suggesting the effectiveness of the morpholino (Supplementary Fig. 4a).
We then tested dependence on LIF for sustained expression.
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