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Transcription analyses systemically, and in an unbiased manner, revealed that the main functions of DMP1 glycosylation was to sustain expression of ECM components including laminins, collagens, integrins, and CSPGs from astrocytes and/or NSCs, and at the same time suppressed cell proliferation, thus allowing astrocytes to stay in a more differentiated functional state.
DIMM cells are dedicated to a very high-level peptidergic function (see below) and so cannot also sustain expression of conventional co-transmitters.
Furthermore, M. Kronenberg and his colleagues recently found that IL-10 secreted by other cells is needed for T reg cells to sustain expression of Foxp3 and prevent colitis [31].
As reported earlier, brefeldin A treatment not only is useful to retain cytokines within the cell cytoplasm, but also to sustain expression of any induced cells surface molecules [15].
Recently, it was reported [12] that adenoviral vector-mediated expression of transcription factor Neurogenin3 (NGN3) in liver resulted in a sustain expression of insulin in neo- islets cells derived most likely from the hepatic progenitor oval cells that secrete insulin in a glucose-responsive manner, stably reversing the hyperglycemia.
Ectopic expression of MycT58A was also able to sustain expression of CD71 and transferrin uptake in IL-2-deprived CTL.
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Three out of the four HCpro mutants suppressed RNA silencing more efficiently and sustained expression of co-expressed jellyfish green fluorescent protein for a longer time than wild-type HCpro in agroinfiltrated leaves of Nicotiana benthamiana.
Cancer cells that express oncogenic alleles of RAS typically require sustained expression of the mutant allele for survival, but the molecular basis of this oncogene dependency remains incompletely understood.
As a result, the sustained release of DNA from modified foams led to negligible cytotoxicity and sustained expression of DNA which is favorable for DNA delivery and tissue engineering application.
Both of these properties are crucial for achieving sustained expression of the transgene.
Lentiviral vectors transduce both dividing and non-dividing cells and can support sustained expression of transgenes.
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