In order to confirm previous findings, we incubated bone-marrow-derived DCs (BMDCs) with fluorescent TRITC-NPs for 30 min. Flow cytometric analysis of BMDC suspensions demonstrated efficient uptake of TRITC-NPs compared to untreated cells.
Test peptides were added to the bacterial suspension after the dye uptake was maximal.
In experiment 1 (CH3Hg+ uptake), the suspensions were tagged with 2.5 μg CH3Hg+ or CH3Hg+ and in experiment 2 (Hg2+ uptake), with 34 μg of Hg2+ or Hg2+.
Although Co2+ ions and cobalt-serum cause limited catalytic oxidation of fresh serum, most of the oxygen uptake by suspensions of metallic cobalt in serum, or by more simple model systems, is due to absorption of oxygen by the metal powder; the consequences of this are discussed.
In contrast, in A.t. Col-0 suspension cells we observed massive uptake of the dye after 20 min of FM 4 64 incubation and many BFA compartments were formed (Fig. 3c and f).
Ca2+-dependent Fe/glyphosate cotransport, which was demonstrated in C. roseus, was found to occur with different degrees of efficiency in various plant cell suspensions, indicating that this glyphosate uptake process can be considered to be a general mechanism in plant cells.
In a recent paper [11], we introduced a mechanistic mathematical model of mitochondrial Ca2+ uniporter (presented briefly in Materials S1) that satisfactorily describes the available experimental data on the kinetics of mitochondrial Ca2+ uptake, measured in suspensions of respiring mitochondria isolated from rat hearts and rat livers under various experimental conditions [12], [13], [16].
Biokinetic models of metal accumulation currently lack estimates of uptake from deposit vs suspension feeding.
Earlier studies in foliar cell suspension cultures indicate that the preferred uptake form of AsA is as the oxidized form, DHA [ 13, 36].
For uptake assays, the vesicle suspension (10 mg lipids/ml) in PBS was incubated with MPP+ or MPDP+ for 2 h at 37°C.
