Exact(4)
Two types of poultices, selected after the investigation of a number of raw materials, whose combination showed desired activities, were combined with a Pseudomonas stutzeri suspension to yield effective bioactive poultices for reducing the nitrate content in the model bricks.
Each tube was inoculated with M. smegmatis cell suspension to yield 104 cells/tube and incubated at 30°C for 72 h.
Briefly, 50 ml of 2 × TY with 100 μg/ml ampicillin and 1% glucose (2 × TY-amp-glu) were inoculated with enough bacterial suspension to yield an OD600 nm ≅ 0.1.
Briefly, 50 ml of 2xTY with 100 μg/ml ampicillin and 1% glucose (2xTY-amp-glu) were inoculated with enough bacterial suspension to yield an OD600 nm≅ 0.05 0.1.
Similar(55)
The appropriate volumes of the enzymatic sample were added directly to the cell suspensions to yield DOX concentration varying in the ranges of 0.1 100 n M, 5 n M 3 μ M and 5 n M 5 μ M for HL60, HL60/VINC and HL60/DOX cells, respectively.
Equal volumes of alginate solution and human articular chondrocyte suspension were combined to yield suspensions with final cell densities of 2 × 107 cells/ml in 1.5% (w/v) alginate.
Subsequently, the tissue was dissociated by incubation with 0.25% trypsin at 37 °C for 15 min, and the resultant tissue suspension was triturated to yield a single cell suspension.
The suspension was filtered to yield an orange solution and the solvent was removed under reduced pressure to yield an orange solid.
Equal volumes (100 μL) of BTE solution and a virus suspension, containing virus to yield 20 30 plaques per well, were placed in microcentrifuge tubes, and the mixtures were incubated at 37°C for 1 h.
Extract solutions were then removed and virus suspensions containing virus to yield 20 30 plaques per well were added to each of the wells.
Finally, the multilamellar vesicles are downsized by extrusion of the lipid suspension through a polycarbonate filter of well-defined pore size at elevated temperature or by sonication to yield a suspension of liposomes with a narrow and well-defined size distribution.
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