The bacterial strains were grown in nutrient broth at 37 °C until the bacterial suspension reached 1.5 × 108 CFU/mL.
The suspension was filtrated and rinsed with deionized water until the pH of the suspension reached about 6, and it was then dried at 80°C.
The electrical conductivity of the 20-nm particle suspension reached 9.60 μS/cm at 7% volume fraction, representing a nearly 100-fold increase over the base fluid.
In addition, the yield of the product in the synthesis system containing seed suspension reached 97%, 15% higher than that obtained in the corresponding synthesis system without the seed suspension.
The suspension was filtrated, and then dialyzed by dialysis bag for 2 weeks, and rinsed with deionized water until the pH of the suspension reached about 6, and then was dried at 80°C.
The kinetic experiment started by switching on the light source after the spectral signal of the Orange II in the suspension reached a steady state; this actual initial signal was taken as a measure of the initial concentration of the dye.
The suspension reached equilibration at 25 °C for 24 h for stirring.
The transformed cells were grown in LB-medium until the OD750 of suspension reached 0.5.
PSI was precipitated in excess methanol and successively washed with deionized water until the pH of the suspension reached neutral.
This process was maintained until a concentrated SpHL-UA suspension reached the final volume of 25 mL.
