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Conidial suspension of each isolate was inoculated on the 2 central spikelets of heads.
A 100 μl suspension of each tested strain was spread uniformly onto SDA plates.
The splenocyte suspension of each group was planted in a 96-well flat bottom plate in 100-μl aliquots.
A 1 10 (w/v) suspension of each sample with distilled water was prepared for pH and electrical conductivity (EC) determination.
Then, 5 ml of bacterial suspension of each strain was added to the RW samples and completely mixed in order to make uniform samples.
Three new pigment morphologies, based on natural ground calcium carbonate (gcc), with special surface structure modifications, are contrasted with standard gcc by using consolidated tablet blocks made from a suspension of each pigment and chosen mixtures thereof.
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Cells passage was performed every 3 4 days and suspensions of each cell line were produced from confluent cultures using 10× TrypLE™ (gibco® by life technologiesTM, USA).
Monoconidial suspensions of each isolate were adjusted to a concentration of 1.5 × 10 conidia/mL.
Once purity was confirmed, heat-denatured suspensions of each isolate were prepared as described previously [ 10].
Single suspensions of each hMSC line at assorted passages and of the hTERT-MSC lines at passage >30 were obtained.
Plates were inoculated with 0.5 McFarland suspensions of each isolate and imipenem, rifampicin or azithromycin Etest strips applied.
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