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The suspending solution was precipitated for 12 h and the upper supernatant was collected and centrifuged.
The conductivity of the suspending solution was measured according to the method of Arce et al.[ 28].
Indeed, previous studies with particles formulated for oral delivery have employed 1% F127 as a suspending solution, which may have reduced mucoadhesion and increased overall effectiveness of the particles.
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He talks to the researchers who are chronicling the plasticisation of the oceans, a swelling suspended solution of pulverised plastic.
Nanoparticles with short peptides adsorbed to their surfaces often assemble into interesting structures when suspended solution.
The suspended solution was centrifuged; the solids were separated and washed three times with water.
The suspended solution was filtered through a 100 μmol/L strainer and diluted in 50 mL PBS.
The suspended solution of Ce-doped CNPs was reserved to use at concentrations of 10 μM by dispersing in 0.1 M Tris-HCl buffer at pH 5.0.
Then, the cells were resuspended in NR medium and 100 μL cell suspended solution was inoculated into 1.7 mL NR medium containing 50 μg/mL kanamycin and cultivated at 30°C for 24 h.
The suspended solution was precipitated by adding acetone and centrifuged at 14,000 rpm for 10 min. Size separation was performed in a water/ethanol/chloroform solvent mixture by high-speed (8,000 to 10,000 rpm) stepwise centrifugation.
This suspended solution was again centrifuged at 2,000 g for 10 min at 2°C, and the supernatant was utilized to determine the activity of GalLDH after cytochrome c was reduced.
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