Normalized samples were prepared by suspending equal amounts of cells from the shake flask experiments in SDS-buffer and loading them on the gel.
The determination of the suspended sediment amount on the rivers is of crucial importance since it directly affects the design and operation of many water resources structures.
That journey requires suspending a fair amount of reality testing, as anyone who has spent time in semi-rural southern France will attest.
(John McClane in "Die Hard" is the arguable exception, though as a workaday cop, he proves to have a disbelief-suspending amount of lethal expertise).
So far, global estimates have only looked at the trash on the surface and not the total amount suspended in water or sitting on the bottom.
For both sperm and control brain samples, equal amount of total protein extract was re-suspended with appropriate amount of lithium dodecyl sulfate (LDS) loading buffer (Invitrogen, Carlsbad, CA, USA).
The reader, at times, has to suspend a considerable amount of disbelief.
It has condemned the putsch, called for the restoration of democracy and suspended a modest amount of military aid to its old ally.
Still, I had to suspend a fair amount of disbelief that this woman had a decades-long legal career of "taking on the state when it behaves badly", three grown children and a cottage in Cornwall.
The samples swelled from the second step were transferred into another three-neck flask of 250 ml and suspended in proper amount of distilled water.
Factors affecting DO are temperature, volume and velocity of water flowing in the river, organic wastes, climate/season, type and number of organisms in the water body, altitude, dissolved or suspended solids and amount of nutrients in the water.
