Exact(16)
Cleared lysate was concentrated by centrivap and suspended in either alcohol or water as described.
Intaglio, lithography and relief inks consist of pigments suspended in either linseed oil or water as a vehicle.
Briefly, GPCs or glioma cells (5 × 104) suspended in either DMEM/F12 medium or DMEM containing 5% FBS were added to the top chamber.
In Experiment I, intestinal organoids were seeded onto PGA mesh discs, suspended in either Matrigel (n = 8) or a vehicle control (n = 8), and implanted into syngenic recipients.
All six were suspended in either April or early May for allegations of expressing anti-Muslim sentiments.
Cells were rapidly harvested and pellets were suspended in either acid (0.2 M HCl) or base (0.2 M NaOH) for NAD or NADH, respectively.
Similar(44)
Overnight cultures of the mentioned bacterial strains were washed twice and re-suspended in either 10 mM phosphate buffer, pH 7.4, and further diluted to 105 cfu/ml.
To remove the non-encapsulated drug, the liposomal suspension was ultracentrifuged at 70 000 g at 4°C for 16 h, and the resulting pellet was re-suspended in either 10 ml of culture media or 10 m M carbonate buffer (pH 7.4), depending on its use (e.g. in vitro or in vivo).
After centrifugation, cell pellets were re-suspended in either 0.03% bovine serum albumin (BSA; w/v) sterilized by membrane filtration or, to represent heavy soiling, 0.3% BSA (w/v) and 0.3% "packed" sheep erythrocytes (v/v), which were prepared as follows.
The pellet was re-suspended in either 4 M urea or 30% formic acid and adjusted to pH 7 with 1 N NaOH and centrifuged at 10,000 × g for 20 min at 4°C, and the supernatant containing the insoluble fraction was collected.
After drug treatment, the cells were washed twice with PBS, re-suspended in either 4 ml lysis buffer (0.3 M sucrose, 0.05 mM EGTA pH 8.0, 60 mM KCl, 15 mM NaCl, 15 mM HEPES pH 7.5, 150 μM spermine, 50 μM spermidine) containing 20 μl triton X-100 for nuclear isolation or 400 μl of 50% ethanol in 1 M HCl (v/v) for whole cell lysis.
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