Sentence examples for suspend cells from inspiring English sources

To suspend cells, the 1.5 mL of fresh medium with TEG was then added to culture dish.

Wash cells twice with DMEM plain medium, suspend cells in blocking medium and then carefully agitate the cells at 37°C for 45 min. Culture human bladder carcinoma ECV 304/T24 cells in cell culture medium until 100%% confluence.

Wash cells twice with DMEM plain medium, suspend cells in blocking medium and then carefully agitate the cells at 37°C for 45 min. Reagents: Blocking buffer (PBS/heat denatured BSA).

Glycerol stocks of adaptation cultures were prepared regularly for subsequent isolation of improved strains or use in reinoculating continuing hydrolyzate dilution series by mixing 200 μL of the greatest hydrolyzate concentration colonized with an appropriate glycerol solution to suspend cells in 10% glycerol in cryovials for freezing at −80°C.

Surface expression of the M-CSF receptor c-fms could not be confirmed by flow cytometry (data not shown), suggesting either that the extensive trypsin treatment to suspend cells degraded the molecule to a form no longer recognized by the detection antibody or that the expression was below the limits detected by flow cytometry.

This established the proper height for dispensing suspended cells.

The suspended cells were disrupted using a high-pressure homogenizer (APV, Denmark) at 900 1000 bar.

In the present study, jute fiber was used to immobilize suspended cells of G. biloba.

The adherent monolayer cells were scratched by using a micropipette tip and washed twice with PBS to remove suspended cells.

The method based on template matching was used to successfully identify sperm cells [17] and other suspended cells [18].

Biofilm cells underwent a phenotypic change, expressing a filamentous cell morphology different from rod-like morphology of suspended cells.