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In summary, the combination of cell culture and real-time RT-PCR detection of early, highly expressed viral genes permits detection of minute quantities of infectious poxvirus particles in a suspected sample.
A suspected sample was subjected to direct sequencing using the cycling sequencing system (Applied Biosystems, Foster City, CA, USA) and run on Perkin-Elmer ABI-377 automatic sequencer.
Application of the developed procedure resulted in the identification of BMPEA in the suspected sample (data not shown), and consequently, the first case of BMPEA doping was reported (2010).
To find extreme concentrations of compounds that are present, a different detection criterion is needed: possible contaminants are identified as signals of higher intensity in the suspected sample than in the other samples.
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In real-time PCR strong positive fluorescence was obtained in 73% of the suspected samples.
Microbes are usually detected in laboratories by feeding nutrients to suspected samples so they grow and expend.
If the disease is suspected, samples will be taken and sent for analysis by plant health experts.
From pre-mortem patients where sCJD was suspected, samples were included in the study only following post-mortem confirmation of sCJD.
When infection was suspected, samples were tested for circulating endotoxin, β-glucan and Candida protein antigens.
The suspected samples were first screened by RT-PCR for PPR virus.
If PD was suspected, samples were routinely sent for laboratory confirmation using histopathological examination and PCR testing.
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