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In contrast to this marked increase in susceptibility, the rate of prion propagation only doubled for the weakly susceptible clone PD88 and decreased for highly susceptible PK1 cells with a concomitant decrease in cell doubling (Supplementary Fig S2).
Firstly, we selected proteins with a minimum of 8-fold decreased in abundance in the susceptible clone at 6 and 24 h after infection.
Both isolates had high chloroquine IC50 (506 nM and 708 nM, respectively) and high chloroquine IC50 isolate: Pf3D7 (chloroquine susceptible clone) ratio (20 and 27, respectively) (Table 2).
The fact that resistant and moderately resistant Salix clones are more efficient in attracting A. nemorum than the susceptible clone strengthens our plant-centred defence theory and opens up novel opportunities for improved breeding.
persicae, susceptible clone 4106A) were transferred onto leaf disks excised from Chinese cabbage (Brassica rapa var chinensis) and kept hydrated on 15 ml agar plugs in individual plastic pots with vented lids.
Desiree, 36 mM phosphite) sampled 3 (Phi3), 6 (Phi6) and 11 (Phi11) h after phosphite treatment, uninfected late blight resistant clones [ 58 ] cv Sarpo Mira (SMC) and clone SW93-1015 (SWC) and uninfected late blight susceptible clone cv.
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Our data do not support either of these patterns: the most common pre-epidemic clones had intermediate susceptibility, and the highly susceptible clones (i.e., those on the right side of Figure 2) increased in frequency.
For each resistant heterozygote, at least 3 susceptible clones were sequenced in effort to avoid any polymerase mistakes.
The PCR, cloning and sequencing were repeated to avoid any possibility of contamination, and more susceptible clones were sequenced to confirm the first results.
N2a and NCerP cells were infected with the Rocky Mountain Lab strain of mouse-adapted scrapie prions (RML) and E2 CWD prions, respectively, and highly susceptible clones isolated, expanded, and aliquots frozen as previously described [37].
Susceptible clones had relatively larger ZOI, while resistant clones had relatively smaller ZOI.
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