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IgG1 antibodies were lower in surviving mice in comparison with non-surviving mice.
Empirical survival curves were generated by plotting proportion of surviving mice against time of death (hours).
After 15 days, surviving mice were euthanized.
Surviving mice underwent intratracheal instillation of 1.5 × 106 CFU of Pseudomonas aeruginosa 8 days after CLP.
On day 5, surviving mice were killed with pentobarbitone (60 mg kg−1 i.p).
BT ≤28°C identified dying mice with 94% specificity, while a BT ≥35°C identified surviving mice with 100% specificity.
The number of surviving mice was counted to preliminarily evaluate the safety of Sal SE-NPs in vivo.
The number of surviving mice was recorded every six hours.
Surviving mice have been sacrificed, and tumor weight evaluated.
All surviving mice showed less than 0.01% of Vβ14+ cells at day 200 (data not shown).
Surviving mice (n = 3 5) were sacrificed after 44 hours and spleens and livers were collected.
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