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Little by little it vanished from Iran, except for a few surviving adherents who remain to the present day in Yazd and a few other places.
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The HER2-positive cell lines BT474 and SKBR3 were subjected to TRAIL/FLIPi or control siRNA (SCi) for 18 hours and only the surviving adherent cells stained for ALDH1 activity.
Still, the majority of the survived adherent cells were undifferentiated expressing high levels of SSEA-4 and low levels of Gata-4.
To determine viability by an independent method we therefore DAPI-labeled nuclei and quantified the surviving, still adherent cells by high-throughput confocal microscopy.
In contrast, IEC-6 cells transformed by either Tpr-Met, or the Grb2 or Shc docking-specific oncoproteins, displayed potent resistance to anoikis, with more than half surviving under non-adherent conditions.
As previously noted in Tzb-refractory pools, the nuclei of the adherent, autophagic surviving SKBR3 parental cells seemed viable.
Despite this turmoil, and the outright hostility toward Christianity of the emperor Julian the Apostate (reigned 361 363), the church survived, and the adherents of the traditional Roman religion relapsed into passive resistance.
Using this protocol, we consistently observed novel surviving populations that were non-adherent and that demonstrated a propensity to form spherical clusters under hypoxic conditions.
This method has been shown to propagate CSC whereas differentiated cells cannot survive the non-adherent conditions.
The surviving population slowly re-populated the adherent conditions (Additional file 1 Figure S8C) and re-acquired an equivalent proportion of mammospheres to the original untreated population (compare SCi samples, Figure 6B, Treatments 1 and 2).
Shīʿism is the only important surviving sect in Islam in terms of numbers of adherents.
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