Within a day nearly all the surviving target ships had been reboarded.
After the August 10 decision to stop decontamination work at Bikini, the surviving target fleet was towed to Kwajalein Atoll where the live ammunition and fuel could be offloaded in uncontaminated water.
By analysis of in vitro cytotoxicity reactions with peripheral human T cells and MT110 in soft agar for potentially surviving target cells, we identified conditions allowing lysis of apparently all TICs.
On day 3, surviving target HMEC cells were detected using trypan blue exclusion (Fig. 4).
For this purpose, surviving target cells were quantified by incorporation of the gamma-emitting analogue of methionine, 75Sel-methionine, in a 48-h assay which detected both cytotoxic and cytostatic effects.
rTNF-alpha did not only enhance cytotoxic capacity but also conferred cytostatic capacity to rIL2-activated LAK cells which were cytotoxic but unable to inhibit the growth of the surviving target cells.
Briefly, attacker strains of S. marcescens Db10 were mixed with target strains P. fluorescens KT02 or S. marcescens ATCC274 strains KT81 or KT82 at an initial ratio of 5 1 and co-cultured for 4 hr at 30°C, followed by enumeration of viable, surviving target cells by selection on streptomycin-containing media.
M k − 1 and M Γ,k denote the predicted hypothesized track number of the surviving targets and the spontaneous births, respectively.
As can be seen from Eq. (13), in essence, the multi-Bernoulli parameter set for the predicted multi-target density π k|k − 1 is formed by the union of the multi-Bernoulli parameter sets for the surviving targets and the spontaneous births.
In contrast, besides surviving targeted gene-disruption, FKN-null mice do not exhibit overt behavioral abnormalities or macroscopic anatomical alterations.
