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To survive, bacteria must be able to adapt to environmental stresses.
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The number of surviving bacteria was expressed as the logarithm of the ratio (survival ratio) of the number of viable bacteria remaining after exposure to experimental conditions (S) to the number of the initial viable bacteria (S0) (log S/S0).
A warm plastic container becomes a perfect incubator for surviving bacteria.
Furthermore, these surviving bacteria may evolve resistance to both antimicrobials and antibiotics that could be transferred to dangerous pathogens.
Although the procedure is routine, it is common for some of the bacteria to survive it and therefore for infections to re-emerge shortly after treatment.The surviving bacteria are often gathered in the form of what is known as a biofilm.
Surviving bacteria were numerated by pour plated counts in MRS agar incubated at 37°C.
The survived bacteria remaining on the culture were counted after 48 h.
The reduction was believed to be due to the metabolic activity of the surviving bacteria through their glycolysis process.
The effluent was collected and the surviving bacteria cells were enumerated using a modified 'Most Probable Number' method.
The surviving bacteria would "level up" and provide the player with a better challenge during the next round.
The problem with chlorination is that surviving bacteria will utilize sodium metabisulfite for nutrition after dechlorination and therefore it is not an ideal choice to prevent biofouling.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com