The test data are distinguished as three cases, i.e. fully paired failure survival specimens, partial paired plus individual survival specimen(s), and partial paired plus individual failure.
The rapid-salinity-decrease experiment lasted for 140 h, and the survival of specimens was checked 28 times in total in a similar manner to the gradual-salinity-decrease experiment.
After appropriate survival times, specimens were rinsed with fresh Medium-199 and incubated for 60 min in 500 µg/ml thermolysin (Sigma; prepared in Medium-199), at 37°C.
In adenocarcinoma tissue, PEA3 expression in combination with elevated ERK MAP kinase signalling is associated with an advanced disease stage and a reduced survival compared with specimens with ERK MAP kinase signalling without PEA3 or in specimens with PEA3 expression in isolation.
In this purpose, a standard method for assessing the long-term survival of field specimens in the laboratory was developed together with experimental designs for investigating food searching and choosing by larvae.
This method is based on the conclusion that under a given fatigue stress S0 the conditional probability of specimen survival at a given fatigue life N0 is equal to the conditional probability that the residual strength of specimens at the fatigue life N0 is greater than S0.
(2) From the parameters, the hazard for each specimen was calculated and the specimen's survival curve was determined.
Different fatigue criteria were used to calculate local survival probabilities at the specimen surface and in the specimen volume.
survival time varied among specimens from 13 to 20 h, neither increasing nor decreasing according to salinity.
