Cells used for the 10-day survival analysis were seeded in several 12-well plates with 8,000 cells per well (~2100 cells/cm2) in normal medium.
Endpoints considered for the survival analysis were loss to follow-up, death, transfer, or study end.
Alternatively, mice intended for survival analysis were monitored by inspection twice daily and euthanized if they appeared to be in pain or moribund.
To determine whether changes in class I HDAC expression were relevant to the recurrence of HCC patients treated with LT, univariate and multivariate survival analysis were performed.
When age was used as a continuous variable, the interaction terms on survival analysis were HR = 1.25 per year (95%CI 0.9 1.7, p = 0.16) and 1.55 (1.2 2.1, p = 0.003) for the effect of ITN use and village respectively.
T-student Test for assessing the significance of in vitro experimentation; Wilcoxon's rank sum test was used to assess the differences in the expression of cystatin C between benign and malignant specimens; Kaplan Meier survival analysis were performed.
Patients alive at the final survival analysis were censored using the last contact date.
All patients in the survival analysis were treated with curative intent.
The entry data for survival analysis were the time of surgery for the primary tumour.
Univariate and multivariate survival analysis were calculated according to Cox's proportional-hazards model.
Variables with P < 0.20 in univariate survival analysis were included in the model.
