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In the surveyed specimens, the minimum number of repeats was 3.2 in CRs of A. modesta, and the maximum number was 10 in CR of B. stellaris although heteroplasmy was present in this case (Additional file 3).
However, we speculate that our results do not represent true endosymbiont losses, but instead, may be due to methodological difficulties associated either with quality/quantity of endosymbiont DNA present in the surveyed specimens (these were small specimens, and had been preserved in ethanol for approximately one year before endosymbiont DNA extraction was conducted), or with PCR amplification.
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Curious to see if there were any general patterns to the depth ranges of coral species, Muir surveyed museum specimens and graphed the depths at which 104 species of staghorn coral had been collected at various latitudes.
We surveyed cranial specimens from adult individuals of the ceratopsid dinosaurs Triceratops and Centrosaurus for bony lesions (see Materials and Methods).
Note: Host voucher code provided only for surveyed planthopper specimens from which new endosymbiont data were generated.
All the spirit-preserved material of extra-clade C Bulbophyllum surveyed (185 specimens in total) revealed only individuals possessing the conventional (Type I) rostellum (see Supporting information, Table S1).
To allow for a broader biochemical survey, specimens of additional hyperoliid species were collected in Cameroon (genera Afrixalus and Hyperolius) and Madagascar (Heterixalus) in the framework of other research projects, and fixed using the same protocols as in Kibale Forest.
10 A fluorescence resonance energy transfer/melt curve analysis (FRET/MCA) 10 was used for quality control of the AS-PCR on a sample of the longitudinal survey specimens (N = 290).
It may thus be that when kept at ambient temperatures, as expected in a countrywide anti-tuberculosis drug resistance survey, specimens change in such a way that cross-reacting substances are generated.
The three men often went off on their own to photograph or sketch as the rest of the party mapped, surveyed and collected specimens.
Invertebrates were surveyed extensively and specimens were distributed to universities and museums for analysis and identification, resulting in four volumes on species ranging from ribbon and segmented worms to sea stars and sea spiders [265], [266], [267].
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