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The total surveyed sequence length was 28.5 kb, excluding two un-amplifiable sequence gaps of 487 bp and 263 bp.
Adjusting the surveyed sequence length to 50 bp and 200 bp yielded similar but less robust results.
We surveyed sequence diversity in the two parental and three hybrid Helianthus species by amplifying a 520-bp region of the Ty3/gypsy-like rt domain-encoding region with degenerate primers followed by cloning and sequencing 92 to 108 amplification products per species.
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The surveyed sequences spanned 43,675 kb and contained almost all of the coding sequences of CYP3A4, CYP3A5, CYP3A7, and CYP3A43.
In order to survey sequence variation in 5S-NTS, random clones, 7 (Paphiopedilum niveum) or 8 (all others) per species, were sequenced (Additional file 1).
The nine-spined stickleback transcriptome characterized in this study allowed us to survey sequence divergence between two closely related species – nine- and three-spined sticklebacks.
With the BVMO-specific sequence motif it has become easy to survey sequenced genomes for BVMO-encoding genes (Fraaije 2002).
Of these, 45 were from BAC-end, fosmid or survey sequencing projects and the rest were from "complete" genome sequencing projects.
Italian Survey Sequence (ca. 12 units): ITALLIT 127, 128, 129.
A next generation sequencing platform (Ion Torrent PGM™) was used to generate a partial genome survey sequence (GSS) dataset to develop microsatellite markers from R. kanagurta genomic DNA.
The following GenBank databases were searched: non redundant, EST, genome survey sequence, high-throughput genomic sequence, patent, whole genome shotgun, sequence tagged site and environmental sequences.
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