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Scoring AFLP polymorphisms in H. erato broods allowed us to survey loci at approximately 362 kb intervals across the genome.
Already, custom arrays, such as Metabochip and Immunochip, utilize SNPs identified through population-based sequencing efforts such as 1000 genomes to better survey loci known to underpin variation across related phenotypes (Trynka et al., 2011).
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These results indicate that exonic sequence deletion and new intron insertion co-occur at a subset of the surveyed loci.
The fact that only a minority of surveyed loci revealed deviations from HWE indicates that the investigated population overall is in Hardy-Weinberg equilibrium, and that deviations at individual loci are most likely due to locus-specific effects, and not due to biological factors such as inbreeding or genetic drift, which would affect all loci.
The same search strategy was applied to other established introns in the same gene for each surveyed locus.
A systematic survey of loci carrying retrotransposons in the genome of the rice blast fungus Magnaporthe grisea allowed the identification of novel non-canonical retropseudogenes.
Much work remains to be done but this data set provides a first-pass list of potential targets for future functional study, and moreover, it provides an initial survey of loci that may have played an important role in the tiger swallowtail radiation.
Mobile genetic elements were found to associate with all SMR homologues at a frequency of 22% within a 10 ORF radius of surveyed genomic loci (Table 2) and upon randomly expanding our genomic loci survey to a 25 ORF radius we saw an increase in mobile element detection (~34%) (data not shown).
Sampling information for the eight individuals used in CAG-tag screening followed by the 126 individuals analyzed in the broader survey of locus transferability.
We surveyed 7 loci and found an average of 0.32 host-specific alleles in 81 STs other than ST-21 that were members of ST-21 complex, i.e., ≈5% of the alleles in this analysis.
TE insertions surveyed by loci 11, 89 and 81 were dated at 3.7 ± SE 0.54 million years ago (hereafter MYA), 2.5 ± SE 0.31 MYA and 1.5 ± SE 0.12 MYA, respectively (Charles et al. 2008), and thus happened after the divergence of the wheat B genome from the U, M, N and C Aegilops genomes (i.e. estimated at 3.5 ± SE 0.5 MYA; Dvorak and Akhunov 2005; Salse et al. 2008).
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