Exact(3)
We cannot formally exclude the possibility that surveillance substrates initially have longer tails that are truncated to the observed lengths.
Conversely, Figure 1G shows that most of the oligo(A) tails present on RNA surveillance substrates are too short to be recovered by oligo dT) selection or to act as primers for oligo dT) primed cDNA synthesis.
Notably, this result implies that the RNAs identified here as surveillance substrates would predominately be overlooked in microarray analyses that involve oligo dT) selection or priming for cDNA synthesis.
Similar(57)
Subsequently, a composite lumenal and/or membrane-associated surveillance system targets ERAD substrates for retrotranslocation and degradation.
Furthermore, experiments using wild-type and mutant alleles of α1AT have been instrumental in uncovering the surveillance and scrutiny that substrates undergo during every step of GQC.
10.7554/eLife.03032.008 Figure 3. PTC-containing APC mRNAs are natural substrates repressed by miRNA-mediated surveillance.
136, 137 Over the last 50 years, filter paper has gained an increasingly important role as a substrate for the diagnosis and surveillance of infectious diseases.
Programmed death receptor is found on the surface of T-cells and its stimulation leads to T-cell deactivation, thus allowing escape from the immune system surveillance in the presence of otherwise antigenic substrate.
This indicates that the dephosphorylation of Cdk1 substrates causes the direct or indirect dissolution of mitotic checkpoint surveillance and highlights cyclin B as a key APC/CCdc20 substrate for this regulation at the metaphase-to-anaphase transition.
Electric cell-substrate impedance sensing (ECIS), a non-invasive, real-time surveillance system, had been used to detect changes in organ cell layer thickness through quantitative monitoring of the impedance of a cell-to-microelectrode interface over time.
Hit clusters and oligoadenylated fragments were recovered at multiple sites on many transcripts, suggesting that repeated rounds of surveillance factor binding and oligo(A) addition may be needed for complete substrate degradation.
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