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Hamster gracilis arteries were rapidly dissected from surrounding skeletal muscle tissue, cannulated and cultured for 24 h under constant flow conditions (1 ml h−1) (ref. 29).
Capillary networks surrounding skeletal muscle play an important role in not only supplying oxygen and nutrients but also in regulating the myogenesis and repair of skeletal muscle tissues.
Once the animals had been killed, a section of gracilis muscle was removed; resistance arteries were dissected from surrounding skeletal muscle tissue, cannulated and functionally assessed by perfusion myography.
Surgeons directly provided a small piece of human intrathoracic fascia-muscle (CABG surgery) or paraspinous muscle (lumbar) to research staff inside the operating room; these biopsies (1 2 cm2) were collected without cautery, and gradually cooled in buffer to 4 °C before the resistance arteries were dissected from the surrounding skeletal muscle tissue.
In humans, the majority of studies demonstrate an age-associated reduction in the number of capillaries surrounding skeletal muscle fibers; however, recent reports in rats suggest that muscle capillarization is well maintained with advanced age.
An intraarterial injection of 5-HT (1 mg/kg) into either conscious or anesthetized (Nembutal, 20 mg/kg) animals resulted in a 53% reduction in tumor blood flow, while that of the surrounding skeletal muscle remained unchanged.
Similar(39)
The technology surrounding temporary skeletal anchorage devices has improved in leaps and bounds.
"Do you have enough food?" she asks one woman surrounded by skeletal and stunned-looking boys and girls.
They've removed my straightjacket, and I'm enjoying a cold drink on my front porch swing, surrounded by skeletal corpses of gardens gone by.
Conversely, a novel result of the present study was the up-regulation of genes encoding type IV collagen, the major structural component of basement membrane surrounding and supporting skeletal muscle cells, and PLOD3, an enzyme playing an essential role in the supramolecular assembly of collagen IV [ 37].
Sr/Ca variability across skeletal features is less than 5% (2σ relative standard deviation) and variability of Sr/Ca within the optically dense central band, composed of small and irregular aragonite crystals, is significantly less than the surrounding skeleton.
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