c Surgical sample obtained after orchiectomy.
e Surgical sample obtained after resection.
e Surgical sample obtained after partial penectomy.
The surgical sample sent for pathological examination was composed of the adrenal gland and of a nodular mass of 4.5 cm in its greater diameter, with a hemorrhagic and partially necrotic cut surface.
Primary glioblastoma cells were prepared from a surgical sample of glioblastoma multiforme.
Additionally, we found the expression of SK1 and SK3 in the surgical sample that was not present in the glioma cell lines (Fig. 1).
Multiple areas of invasive carcinoma and different histological patterns of the tumours (cribiform, mucinous, poorly-differentiated), adenomatous lesions from the same surgical sample, and normal mucosa, located far from the infiltrating tumour, were identified on corresponding haematoxylin-eosin-stained slides.
Since immortalized cell lines frequently have different gene expression profile from cells in situ and primary cell cultures, we performed recordings in primary glioma cultures prepared from a human surgical sample of GBM.
In fact, we previously reported that biopsy sample expression profiles could distinguish long-term or short-term survivors by definitive chemoradiotherapy [8]; however, surgical sample expression profiles never identified poor prognostic subgroups with extensive lymph node metastasis [10].
In order to determine if glioma cells in situ have a similar Ca2+-activated K+ channel expression profile, we isolated mRNA from a surgical sample that was histopathologically identified as a GBM.
