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The cartilage was exposed with the help of a spatula and a cylindrical defect (3 mm in diameter and 2 mm thick) was created at the caudal end of the xiphoid cartilage with a surgical punch.
Skin biopsies with gross pathologic changes were collected with a surgical punch 5 mm in diameter (Miltex Stainless, Germany) and stored at −80°C for virus isolation and PCR analysis.
Position one thumb at either side of the surgical punch (cut/slit), and gently push down (careful not to break or fracture any toes or tiny bones).
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Liver tissue was transported to the laboratory within approximately 30 min and cylindrical liver cores were produced using a surgical biopsy punch with diameter of 5 mm (KAI, SynErgo Europe, Romania).
Next, 5 mm cylindrical liver cores were prepared with a surgical biopsy punch and sectioned to 200 μm slices using a Krumedieck tissue slicer (Alabama Research and Development, Munford, AL, USA) filled with carbonated KHB.
Donor characteristics of the livers used for slicing are shown in Table 1.> PCLS were prepared and cultured as described previously [ 31]. 5 mm cylindrical liver cores were prepared with a surgical biopsy punch and sectioned to 200 μm slices using a Krumdieck tissue slicer (Alabama Research and Development, Munford, AL, USA) filled with carbonated KHB (pH 7.4, supplemented with 25 mM glucose).
Evaluation of suspicious skin lesions by dermatologists is usually accomplished using white light examination and direct punch or surgical biopsy.
Two cores were taken from each surgical sample by using punch cores that measured 1.5 mm in the greatest dimension from the centre of the tumour foci.
Putting the Social Safety Net on the Operating Table is not a "Punch the Hippie" Surgical Procedure as much as it is something else.
The skin biopsy specimens were taken from one papulopustule with a punch or a surgical knife into a dry tube, usually by a local physician.
Subcision and punch excision remain important surgical options for certain types of atrophic acne scars.
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