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Proteins (insulin, ubiquitin and lysozyme) were incubated with these surfaces at various pH levels, and the protein adsorption capability of the surfaces was quantified using matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS).
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The texture and in-plane biaxial residual stress of the treated surfaces were quantified using contact profilometry and X-ray diffraction, respectively.
The presence of free amino groups on the aminolyzed PLLC surface was quantified using fluorescamine analysis method, which revealed that the surface NH2 density increased and eventually saturated with increasing 1,6-hexanediamine concentration or reaction time.
The thickness of the biopolymer brush layer on the bacterial surface was quantified using a steric model, and these values increased as polarity increased, with values of 27, 93, and 257 nm in methanol, water, and formamide, respectively.
The number of NH2 groups present on the functionalised contact lens surface was quantified using a methyl orange assay.
In the surface randomly selected by cross-sectioning through the simulated pore structure, the stress and strain fields around each pore in the surface were quantified using a 3-D finite element model under an applied cyclic stress, and the fatigue crack incubation life at the pore was estimated with a micro-scale Manson-Coffin equation.
Whole aorta surface area was quantified using ImageJ software.
The corresponding surface roughness was quantified using root mean square (RMS) average of height deviations parameter.
After three PBS washes, each substratum was observed using an inverted fluorescent microscope (Olympus Corp .. Fluorescence intensity per surface unit was quantified using NIH Image software (Image J, http://www.rsb.info.nih.gov).
The intactness and chemical functionality of the inactivated virions was then assessed by size-exclusion chromatography, transmission electron microscopy, and the surface reactivity was quantified using N-hydroxysuccinimidyl ester conjugation of a fluorophore.
Expression of cell surface antigens was quantified using flow cytometry.
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