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The importance of each patch as an indicator for the connectivity of forest surfaces was determined using a binary index, the Harary index (dH).
Quantification of CD70 copy number on the cell surfaces was determined using a murine anti-CD70 mAb (Ki-24, BD Pharmingen) and the DAKO QiFiKit flow cytometric indirect assay as described by the manufacturer (DAKO A/S, Glostrup, Denmark).
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The contour lengths of single DNA molecules and percentage of DNA occupied on surfaces were determined using METAMORPH software (MDS , Inc.
In addition, residual stresses at the surfaces were determined using X-ray diffraction and the sin2ψ method.
Potential energy surfaces were determined using 10 different approaches, including state-of-the-art DFT (M06, M06-2X and M08-SO) and contemporary composite methods (G4, G4MP2, CBS-QB3 and CBS-4M).
The width and height of EAK16-II nanofibers on mica and HOPG surfaces were determined using the cross-sectional analysis tool of the PicoScan software.
The amounts of rhBMP-2 released from surfaces were determined using an ELISA kit and a microplate reader (Bio-Rad, Hercules, CA, USA) at 450 nm.
The ratio of orthoclase phenocrysts (ROP) on the failure surface was determined using image analysis.
The percentage of PAMAM that grafted onto the silica surface was determined using a previously described equation [14].
The cleft between the neuron cell membrane and the surface was determined using fluorescence interference contrast (FLIC) microscopy.
The percentage oxidation of the surface was determined using X-ray photoelectron spectroscopy (XPS), and surface stoichiometry was measured using scanning electron microscopy - energy dispersive X-ray spectroscopy (SEM-EDS).
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