The presence of free amino groups on the aminolyzed PLLC surface was quantified using fluorescamine analysis method, which revealed that the surface NH2 density increased and eventually saturated with increasing 1,6-hexanediamine concentration or reaction time.
The thickness of the biopolymer brush layer on the bacterial surface was quantified using a steric model, and these values increased as polarity increased, with values of 27, 93, and 257 nm in methanol, water, and formamide, respectively.
The number of NH2 groups present on the functionalised contact lens surface was quantified using a methyl orange assay.
The texture and in-plane biaxial residual stress of the treated surfaces were quantified using contact profilometry and X-ray diffraction, respectively.
Proteins (insulin, ubiquitin and lysozyme) were incubated with these surfaces at various pH levels, and the protein adsorption capability of the surfaces was quantified using matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS).
For quantitative analysis, EC nuclei number, tight junction number, pericyte cell body/process length, and EC surface length were quantified using ImageJ software (NIH) on a minimum of six images of cerebral blood vessels per sample.
Surface flow velocities were quantified using particle image velocimetry.
Surface GluA2 puncta were quantified using immunofluorescence microscopy.
The surface changes and wear progress were quantified using conventional surface roughness parameters as well as a set of visual examinations based on a scanning electron microscope and extended it to three-dimensional interpretation.
The effects of varying process parameters, for instance laser power and nitrogen pressure on the surface quality, namely discolouration were quantified using ImageJ analysis.
